Coding

Part:BBa_I756005:Design

Designed by: Andy Mendelsohn   Group: iGEM07_Bay_Area_RSI   (2007-08-10)


IgM signal seq - Vh-linker-VL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
    Illegal XbaI site found at 11
    Illegal SpeI site found at 842
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
    Illegal SpeI site found at 842
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal BamHI site found at 678
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
    Illegal XbaI site found at 11
    Illegal SpeI site found at 842
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
    Illegal XbaI site found at 11
    Illegal SpeI site found at 842
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 507


Design Notes

Fusion in frame of IgM signal sequence in frame with anti-cardiac myosin Heavy chain


Source

Part is to be synthesized

References